PURIFICATION AND CHARACTERIZATION OF ALANINE AMINOTRANSFERASE FROM PANICUM-MILIACEUM LEAVES

被引：30

作者：

SON, DY ^{[1
]}

JO, JK ^{[1
]}

SUGIYAMA, T ^{[1
]}

机构：

[1] KYUNGPOOK NATL UNIV,COLL AGR,DEPT DAIRY SCI,TAEGU 702701,SOUTH KOREA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 289卷 / 02期

关键词：

D O I：

10.1016/0003-9861(91)90470-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the enzyme. The enzyme, with an apparent molecular size of 102 kDa, appeared to be a dimer of a single 50-kDa polypeptide. The enzyme has a relatively broad pH optima with similar curves for the forward and reverse directions, ranging between 6.5 and 7.5. The Km values of this enzyme were 6.67, 0.15, 5.00, and 0.33 mm for alanine, 2-oxoglutarate, glutamate, and pyruvate, respectively. The activity of AlaAT-2 was found to increase markedly during leaf greening in parallel with the increase of immunochemically titrated protein, and it is suggested to function in the C4 photosynthetic cycle. © 1991.

引用

页码：262 / 266

页数：5

共 17 条

[1] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].

ARNON, DI .

PLANT PHYSIOLOGY, 1949, 24 (01) :1-15

[2]

GATEHOUSE PW, 1967, J BIOL CHEM, V242, P2319

[3] IDENTIFICATION OF SOLUBLE AND DISSOLVED FORMS OF ALANINE ALPHA-KETOGLUTARATE AMINOTRANSFERASE FROM MITOCHONDRIA OF LYPERSICON-ESCULENTUM [J].

GAZEUREYJAL, M ;

CROUZET, J .

PHYTOCHEMISTRY, 1976, 15 (11) :1619-1622

[4]

Givan CV, 1980, BIOCH PLANTS, P329

[5] C-4 PHOTOSYNTHESIS - A UNIQUE BLEND OF MODIFIED BIOCHEMISTRY, ANATOMY AND ULTRASTRUCTURE [J].

HATCH, MD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 895 (02) :81-106

[6] SEPARATION AND PROPERTIES OF LEAF ASPARTATE AMINOTRANSFERASE AND ALANINE AMINOTRANSFERASE ISOENZYMES OPERATIVE IN C4 PATHWAY OF PHOTOSYNTHESIS [J].

HATCH, MD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 156 (01) :207-214

[7] ACTIVITY, LOCATION, AND ROLE OF ASPARTATE AMINOTRANSFERASE AND ALANINE AMINOTRANSFERASE ISOENZYMES IN LEAVES WITH C4 PATHWAY PHOTOSYNTHESIS [J].

HATCH, MD ;

MAU, SL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 156 (01) :195-206

[8] LIGHT-MODULATION OF MAIZE LEAF PHOSPHOENOLPYRUVATE CARBOXYLASE [J].

HUBER, SC ;

SUGIYAMA, T ;

AKAZAWA, T .

PLANT PHYSIOLOGY, 1986, 82 (02) :550-554

[9] SEPARATION OF MESOPHYLL PROTOPLASTS AND BUNDLE SHEATH CELLS FROM MAIZE LEAVES FOR PHOTOSYNTHETIC STUDIES [J].

KANAI, R ;

EDWARDS, GE .

PLANT PHYSIOLOGY, 1973, 51 (06) :1133-1137

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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