Mycoplasma virus MV-Lg-pS2-L172 was sensitive to heat (56° C/30 minutes), Nonidet-P40 and ether. In these respects it resembled Plasmavirus MV-L2. However, it differed from MV-L2 (and the other mycoplasma viruses, MV-L1, MV-L3 and BN 1 virus) in reciprocal plaque inhibition and serum neutralization tests (MV-L2 only). By plaque formation on host lawns resistant to the different mycoplasma viruses, including MV-Lg-pS2-L172, this latter virus was shown to be distinct from the other viruses, including MV-L2. Both MV-Lg-pS2-L172 and MV-L2 possessed one polypeptide band (out of 10) that was not common to the heterologous virus. © 1979 Springer-Verlag.