SPECIFIC CHEMICAL LABELING OF DNA FRAGMENTS

被引：31

作者：

ESHAGHPOUR, H

SOLL, D

CROTHERS, DM

机构：

[1] YALE UNIV,DEPT MOLEC BIOPHYS,NEW HAVEN,CT 06520

[2] YALE UNIV,DEPT BIOCHEM,NEW HAVEN,CT 06520

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.6.1485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termin1. The procedure includes enzymatic addition of 4-th1ourid1ne, followed by reaction in mild non-denaturing conditions with the highly reactive α-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with β-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution. © 1979 Information Retrieval Limited.

引用

页码：1485 / 1495

页数：11

共 23 条

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