PROSTANOID-INDUCED INHIBITION OF LIPOLYSIS IN RAT ISOLATED ADIPOCYTES - PROBABLE INVOLVEMENT OF EP3 RECEPTORS

Sulprostone, enprostil and 16,16 dimethyl PGE2 have all been found to be potent inhibitors of lipolysis induced by 3-isobutyl-1-methyl-xanthine (IBMX) in rat isolated adipocytes. The potency of sulprostone and enprostil in particular indicates that the response is likely to be mediated through either EP3 or EP1-receptors. However, the EP1-receptor blocking drug, AH6809, had no effect on the antilipolytic response to either PGE2 or sulprostone. We therefore conclude that the receptors mediating prostanoid-induced inhibition of lipolysis in rat adipocytes must principally be of the EP3 sub-type.