RNA DUPLEX UNWINDING ACTIVITY OF ALFALFA MOSAIC-VIRUS RNA-DEPENDENT RNA-POLYMERASE

被引：9

作者：

DEGRAAFF, M

HOUWING, CJ

LUKACS, N

JASPARS, EMJ

机构：

[1] Institute of Molecular Plant Sciences, Plant Virology Section, Gorlaeus Laboratories, NL-2300 RA Leiden

来源：

FEBS LETTERS | 1995年 / 371卷 / 03期

关键词：

ALFALFA MOSAIC VIRUS; RNA-DEPENDENT RNA POLYMERASE; RNA DUPLEX UNWINDING;

D O I：

10.1016/0014-5793(95)00875-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An RNA-dependent RNA polymerase (RdRp) purified from alfalfa mosaic virus-infected tobacco is capable of synthesizing in vitro full-size RNAs of minus and plus polarities. However, the enzyme is not able to perform a complete replication cycle in vitro. The products were found to be completely base-paired to their templates. The enzyme was able to use double-stranded RNA as a template for RNA synthesis if it could initiate from a single-stranded promoter, The inability (of most) of our enzyme preparations to create a single-stranded initiation site could explain why they could not perform a complete replication cycle in vitro, This is the first report on duplex RNA unwinding activities by a plant viral RdRp.

引用

页码：219 / 222

页数：4

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