METHOD OF REVERSIBLE INACTIVATION OF SMALL REGIONS OF BRAIN-TISSUE

被引:84
作者
MALPELI, JG [1 ]
SCHILLER, PH [1 ]
机构
[1] MIT,DEPT PSYCHOL,CAMBRIDGE,MA 02139
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1016/0165-0270(79)90011-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method is described for reversibly inactivating small, precisely localized regions of brain tissue with injections of nanoliter quantities of the local anesthetic lidocaine hydrochloride. The injections are made through a combined recording-injection probe consisting of a glass micropipette onto whose outer surface is plated a metallic cylinder for recording extracellular action potentials. The recording cylinder, located a known distance from the pipette tip, picks up a continuous, large-amplitude, multiunit response which can be used to accurately position the tip according to physiological criteria. It also provides a means of determining the duration of the anesthetic block and estimating its spread. This devise has been used to selectively block 200-400 μm regions of individual laminae of the lateral geniculate nucleus centered within 50 μm of a retinotopically defined target site. The blocks last from 3 to 10 min and can be repeated many times at the same location. © 1979.
引用
收藏
页码:143 / 151
页数:9
相关论文
共 6 条
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