HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF VANCOMYCIN IN SERUM

被引：41

作者：

UHL, JR ^{[1
]}

ANHALT, JP ^{[1
]}

机构：

[1] MAYO CLIN & MAYO FDN,ROCHESTER,MN 55901

来源：

THERAPEUTIC DRUG MONITORING | 1979年 / 1卷 / 01期

关键词：

Bioassay comparison; HPLC assay; Ristocetin; Vancomycin;

D O I：

10.1097/00007691-197901000-00008

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A liquid chromatographic procedure is described for rapid and accurate quantitation of vancomycin in serum. Vancomycin is extracted from serum by using a CM-Sephadex® column and is measured by reversed-phase chromatography with detection by UV absorption. Ristocetin is used as an internal standard. Concentration and instrument response are linearly related in the range of therapeutically useful concentrations. Results correlate well with those obtained by bioassay. The between-day coefficient of variation (n = 8) was less than 4%. Recovery of vancomycin from serum is nearly complete, and no substances that interfere with the procedure have been detected in clinical specimens. © 1979 Raven Press, New York.

引用

页码：75 / 83

页数：9

共 16 条

[1]

McCormick M.H., Stark W.M., Pittenger G.E., Pittenger R.C., McGuire J.M., Vancomycin, a new antibiotic. I. Chemical and biologic properties, Antibiotics Annual 1955-1956, pp. 606-611, (1956)

[2]

Geraci J.E., Vancomycin, Mayo Clin Proc, 52, pp. 631-634, (1977)

[3]

Geraci J.E., Heilman F.R., Nichols D.R., Wellman W.E., Ross G.T., Some laboratory and clinical experiences with a new antibiotic, vancomycin, Proc Staff Meet Mavo Clin, 31, pp. 564-582, (1956)

[4]

Cook F.V., Farrar W.E., Vancomycin revisited, Ann Intern Med, 88, pp. 813-818, (1978)

[5]

Hande K.R., Witebsky F.G., Brown M.S., Schulman C.B., Erson S.E., Levine A.S., Maclowry J.D., Chabner B.A., Sepsis with a new species of Corynebacterium, Ann Intern Med, 85, pp. 423-426, (1976)

[6]

Kirby W., Divelbiss C.L., Vancomycin: Clinical and laboratory studies, Antibiotics Annual 1956-1957, pp. 107-117, (1957)

[7]

Lindholm D.D., Murray J.S., Persistence of vancomycin in the blood during renal failure and its treatment by hemodialysis, N Engl J Med, 274, pp. 1047-1051, (1966)

[8]

Hook E., Johnson W.D., Vancomycin Therapy of Bacterial Endocarditis, 212, (1976)

[9]

Geraci J.E., Heilman F.R., Nichols D.R., Wellman W.E., Antibiotic therapy of bacterial endocarditis. VII. Vancomycin for acute micrococcal endocarditis. Preliminary report, Proc Staff Meet Mayo Clinic, 33, pp. 172-181, (1958)

[10]

Wilson W.R., Van Scoy R.E., [Letter.] Mayo Clin Proc, 53, pp. 197-198, (1978)

← 1 2 →