ANTIBODY TO B-SUBTILIS DNA POLYMERASE-III - USE IN ENZYME-PURIFICATION AND EXAMINATION OF HOMOLOGY AMONG REPLICATION-SPECIFIC DNA-POLYMERASES

Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B.subtilis pol III. © 1979 Information Retrieval Limited.