THE SULFHYDRYL-GROUPS RESPONSIBLE FOR BILITRANSLOCASE TRANSPORT ACTIVITY RESPOND TO THE INTERACTION OF THE CARRIER WITH BILIRUBIN AND FUNCTIONAL ANALOGS

被引:14
作者
PASSAMONTI, S [1 ]
SOTTOCASA, GL [1 ]
机构
[1] UNIV TRIESTE,DIPARTIMENTO BIOCHIM BIOFIS & CHIM MACROMOLEC,VIA A VALERIO 32,I-34127 TRIESTE,ITALY
关键词
(Rat liver); Bilirubin; Bilitranslocase; Electrogenic transport; Sulfhydryl group; Sulfobromophthalein;
D O I
10.1016/0005-2736(90)90376-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Both inactivation of sulfobromophthalein transport in rat liver plasma membrane vesicles by sulfhydryl group reagents and subsequent reactivation by 2-mercaptoethanol are shown to be modulated by ligands to bilitranslocase. In particular, bilirubin, sulfobromophthalein and Thymol blue behave as negative effectors in the inactivation reaction and as positive effectors in the reactivation reaction. Kinetic data provide further evidence of the existence of two classes of sulfhydryl groups involved in transport activity. The effect brought about by remarkably low concentration of bilirubin is in line with the physiological function of bilitranslocase as a bilirubin carrier. © 1990.
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页码:9 / 12
页数:4
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