INVOLVEMENT OF GANGLIOSIDES IN LYMPHOCYTE STIMULATION

被引：58

作者：

SPIEGEL, S ^{[1
]}

RAVID, A ^{[1
]}

WILCHEK, M ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT BIOCHEM,REHOVOT 76100,ISRAEL

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 10期

关键词：

D O I：

10.1073/pnas.76.10.5277

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

N4-Dinitrophenyl-L-2,4-diaminobutyric acid hydrazide was attached covalently to the aldehyde groups produced by periodate oxidation of bovine brain gangliosides and it was incorporated into mature thymocytes. High concentrations of antibodies to dinitrophenyl (DNP) or lower concentrations followed by protein A agglutinated the cells and stimulated DNA synthesis. Incubation of the thymocytes containing DNP-modified ganglioside with rabbit anti-DNP and fluorescein-labeled anti-rabbit IgG resulted in the formation of uniform rings on the cell surface, which subsequently developed into patches and caps. These results indicate that gangliosides may play a primary role in the lymphocyte activation process.

引用

页码：5277 / 5281

页数：5

共 25 条

[1]

ALLEN D, 1972, NATURE-NEW BIOL, V236, P23

[2] BINDING OF INTERFERON TO GANGLIOSIDES [J].