TIME-RESOLVED CRYOELECTRON MICROSCOPIC STUDY OF THE DISSOCIATION OF ACTOMYOSIN INDUCED BY PHOTOLYSIS OF PHOTOLABILE NUCLEOTIDES

被引：57

作者：

MENETRET, JF

HOFMANN, W

SCHRODER, RR

RAPP, G

GOODY, RS

机构：

[1] MAX PLANCK INST MED RES,DEPT BIOPHYS,JAHNSTR 29,W-6900 HEIDELBERG,GERMANY

[2] EUROPEAN MOLEC BIOL LAB,OUTSTN,DEUTSCH ELEKTR SYNCHROTRON,W-2000 HAMBURG,GERMANY

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 02期

关键词：

TIME-RESOLVED CRYOELECTRON MICROSCOPY; CAGED NUCLEOTIDES; ACTOMYOSIN; ACTIN;

D O I：

10.1016/0022-2836(91)90554-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rapid release of a substrate or other ligand from photolabile precursors in a thin layer suspension of biological specimens followed by rapid freezing provides a method of trapping and visualizing short-lived states in a dynamic system. We demonstrate here the first successful application of this method to study the interaction of actin filaments with myosin subfragment 1 (S1) after release of nucleotides. The results obtained suggest that structural changes in actin filaments occur as a result of interaction with S1. © 1991.

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页码：139 / 144

页数：6

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