G-PROTEIN G(I2)ALPHA IN THE COCHLEA - CLONING AND SELECTIVE OCCURRENCE IN RECEPTOR-CELLS

被引：13

作者：

TACHIBANA, M

ASANO, T

WILCOX, E

YOKOTANI, N

RIVOLTA, MN

FEX, J

机构：

[1] NIDOCD, NEUROCHEM LAB, BETHESDA, MD 20892 USA

[2] AICHI PREFECTURAL COLONY, INST DEV RES, DEPT BIOCHEM, AICHI, JAPAN

来源：

MOLECULAR BRAIN RESEARCH | 1994年 / 21卷 / 3-4期

关键词：

G(I) PROTEIN; A SUBUNIT; CDNA LIBRARY; MOLECULAR CLONING; IMMUNOHISTOCHEMISTRY; AFFINITY-PURIFIED ANTIBODY; COCHLEA; INNER HAIR CELL; OUTER HAIR CELL;

D O I：

10.1016/0169-328X(94)90267-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A cDNA library was made from the mouse cochlea and screened with a G protein-cDNA like molecule obtained from cochlear tissue by polymerase chain reaction. The nucleotide sequence of a clone, named cochlear G(12)alpha, had 99.2% identity to mouse macrophage G(12)alpha. Using an antibody which is selective for G(12)alpha, expression of the cochlear G(12)alpha was localized in outer and inner hair cells of the organ of Corti. Possible functional roles of this G protein in hair cells are discussed.

引用

页码：355 / 358

页数：4

共 19 条

[1] HEAVY-METAL INTENSIFICATION OF DAB-BASED HRP REACTION-PRODUCT [J].