ABERRANT HISTOPLASMA-CAPSULATUM - CONFIRMATION OF IDENTITY BY A CHEMILUMINESCENCE-LABELED DNA-PROBE

被引:8
作者
SANDIN, RL
ISADA, CM
HALL, GS
TOMFORD, JW
RUTHERFORD, I
ROGERS, AL
WASHINGTON, JA
机构
[1] CLEVELAND CLIN FDN,DEPT MICROBIOL,CLEVELAND,OH 44195
[2] CLEVELAND CLIN FDN,DEPT INFECT DIS,CLEVELAND,OH
[3] MICHIGAN STATE UNIV,DEPT BOT,E LANSING,MI
[4] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
关键词
D O I
10.1016/0732-8893(93)90103-E
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A cottony, light tan, filamentous fungus with pear-shaped microconidia and lacking tuberculated macroconidia was isolated from a bronchial lavage specimen. Subculture on several media at 37 degrees C failed to convert the fungus to a yeast form after several weeks; attempts at in vivo conversion in mice were also unsuccessful. Sera obtained several months apart showed M bands with Histoplasma capsulatum (HC) antigen by immunodiffusion and an increase in complement Fixation titers with mycelial and yeast phase antigens of HC. Parallel identity was obtained on two occasions with exoantigen culture confirmation reagents for HC from Immuno-Mycologics as well as one of identity with Nolan reagents. Extracts from four Chrysosporium spp. strains had no identity reactions with HC with either kit. The fungus was identified as HC by the Accuprobe Histoplasma chemiluminescence-labeled DNA probe directed or ribosomal RNA, whereas all four Chrysosporium spp. isolates tested negative. DNA probes are a fast and accurate method to confirm the identity of aberrant fungal isolates.
引用
收藏
页码:235 / 238
页数:4
相关论文
共 18 条
[1]  
BODY BA, 1988, ARCH PATHOL LAB MED, V112, P519
[2]   EVALUATION OF A CHEMILUMINESCENT PROBE ASSAY FOR IDENTIFICATION OF HISTOPLASMA-CAPSULATUM ISOLATES [J].
HALL, GS ;
PRATTRIPPIN, K ;
WASHINGTON, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :3003-3004
[3]   EVALUATION OF GEN-PROBES HISTOPLASMA-CAPSULATUM AND CRYPTOCOCCUS-NEOFORMANS ACCUPROBES [J].
HUFFNAGLE, KE ;
GANDER, RM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (02) :419-421
[4]   VARIATION IN COMPLEMENT-FIXATION TEST-RESULTS WITH 3 HISTOPLASMA-CAPSULATUM YEAST PHASE ANTIGENS [J].
JOHNSON, JE ;
GOODMAN, NL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (06) :1066-1067
[5]   EXOANTIGEN TESTS FOR THE IMMUNOIDENTIFICATION OF FUNGAL CULTURES [J].
KAUFMAN, L ;
STANDARD, P ;
PADHYE, AA .
MYCOPATHOLOGIA, 1983, 82 (01) :3-12
[6]  
KAUFMAN L, 1987, ANNU REV MICROBIOL, V41, P209, DOI 10.1146/annurev.mi.41.100187.001233
[7]  
MORRIS PR, 1986, J MED VET MYCOL, V24, P231
[8]  
PRATTRIPPIN K, 1991, 91ST ANN M AM SOC MI, P83
[9]   UNPREDICTABILITY OF COMMERCIALLY AVAILABLE EXOANTIGEN CULTURE CONFIRMATION TESTS IN CONFIRMING THE IDENTITY OF 5 BLASTOMYCES-DERMATITIDIS ISOLATES [J].
SANDIN, RL ;
HALL, GS ;
LONGWORTH, DL ;
WASHINGTON, JA .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1993, 99 (05) :542-545
[10]  
SEKHON AS, 1986, DIAGN MICROBIOL INFE, V4, P326