RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF CRINIPELLIS-PERNICIOSA ISOLATES FROM DIFFERENT HOSTS

被引:25
作者
ANDEBRHAN, T
FURTEK, DB
机构
[1] PENN STATE UNIV,AMER COCOA RES INST,DEPT FOOD SCI,MOLEC BIOL LAB,UNIVERSITY PK,PA 16802
[2] PENN STATE UNIV,INST BIOTECHNOL,UNIVERSITY PK,PA 16802
关键词
D O I
10.1111/j.1365-3059.1994.tb01651.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
DNA sequence polymorphisms among isolates of Crinipellis perniciosa, causing witches' broom disease in several genera and species in the Sterculiaceae, Solanaceae and Bixaceae, were revealed by random amplified polymorphic DNA (RAPD) banding patterns. Comparisons were made among isolates from Theobroma cacao (cultivars SCA6 and SVB), T. grandiflorum, T. obovatum, T. subincanum, Herrania sp. (all collected near Belem, Brazil, at three plots within two kilometres of each other), and Bixa orellana and Solanum rugosum (150 and 1440 km from Belem, respectively). Genetic similarities, as determined by Sorensen's coefficient (Sc-ij; fraction of shared bands), ranged from 1.00 (T. cacao SCA6 and T. grandiflorum) to 0.56 (S. rugosum and T. subincanum). Results indicate that proximity is more important than host species for determining the genetic relationships among isolates, that there were two independent sources of inoculum at the site near Belem, and that the isolate from B. orellana is most closely related to the isolate from T. cacao SVB. Comparisons were also made among multispore cultures from different basidiocarps on the same T. cacao SVB broom, as well as monospore cultures from the same S. rugosum basidiocarp. Banding patterns were similar among basidiocarps on the same broom, but differences were detected among monospore cultures from the same basidiocarp.
引用
收藏
页码:1020 / 1027
页数:8
相关论文
共 31 条
  • [1] ANDEBRHAN T, 1988, 10TH INTERNATIONAL COCOA RESEARCH CONFERENCE, P363
  • [2] ANDEBRHAN T, 1985, 9TH P INT COC RES C, P395
  • [3] USE OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS TO DISTINGUISH ISOLATES OF ASPERGILLUS-FUMIGATUS
    AUFAUVREBROWN, A
    COHEN, J
    HOLDEN, DW
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) : 2991 - 2993
  • [4] BAKER RED, 1957, 2 COMM MYC I PHYT PA
  • [5] BASTOS C N, 1988, Fitopatologia Brasileira, V13, P202
  • [6] BASTOS C N, 1986, Fitopatologia Brasileira, V11, P963
  • [7] A NEW PATHOTYPE OF CRINIPELLIS-PERNICIOSA (WITCHES BROOM DISEASE) ON SOLANACEOUS HOSTS
    BASTOS, CN
    EVANS, HC
    [J]. PLANT PATHOLOGY, 1985, 34 (02) : 306 - 312
  • [8] HISTOLOGY OF WITCHES-BROOM CAUSED IN CACAO BY CRINIPELLIS-PERNICIOSA
    CALLE, H
    COOK, AA
    FERNANDO, SY
    [J]. PHYTOPATHOLOGY, 1982, 72 (11) : 1479 - 1481
  • [9] DIFFERENTIATION OF FUSARIUM-SOLANI F-SP CUCURBITAE RACE-1 AND RACE-2 BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA
    CROWHURST, RN
    HAWTHORNE, BT
    RIKKERINK, EHA
    TEMPLETON, MD
    [J]. CURRENT GENETICS, 1991, 20 (05) : 391 - 396
  • [10] NUCLEAR CONDITION OF BASIDIA, BASIDIOSPORES, AND MYCELIUM OF MARASMIUS-PERNICIOSUS
    DELGADO, JC
    COOK, AA
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1976, 54 (1-2): : 66 - 72