DISTRIBUTION OF NEUTRAL ENDOPEPTIDASE ACTIVITY IN HUMAN BLOOD LEUKOCYTES

被引:38
作者
IWAMOTO, I
KIMURA, A
OCHIAI, K
TOMIOKA, H
YOSHIDA, S
机构
[1] 2nd Dept. of Internal Med., Chiba University, School of Medicine, Chiba City 280
关键词
PROTEASE; NEUROPEPTIDES; SUBSTANCE-P; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN (CALLA);
D O I
10.1002/jlb.49.2.116
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We investigated the distribution of neutral endopeptidase (NEP; EC 3.4.24.11) activity, a possible regulatory enzyme for neuropeptide-induced leukocyte activations, in each cell type of human blood leukocytes. The NEP activity assessed by an NEP inhibitor phosphoramidon-sensitive Met5-enkephalin degrading activity was present in neutrophils and the common acute lymphoblastic leukemia antigen (CALLA)-positive leukemic cells (59 pmol/min/10(6) cells and 62 pmol/min/10(6) cells, respectively); however, the NEP activity was virtually absent in lymphocytes, monocytes, eosinophils, basophils, CALLA-negative leukemic cells, or a promyelocytic cell line HL-60. The enzymatic activity was characterized as NEP on the basis of the values of kinetic parameters (K(m) = 61-mu-M, K(cat) = 1,692 min-1, and K(cat/K(m) = 28 min(-1)mu-M-1) and the values of IC50 of two NEP inhibitors phosphoramidon and thiorphan (7.4 nM and 8.4 nM, respectively). The distribution of NEP detected immunocytochemically using anti-NEP monoclonal antibodies was also found to be parallel with the distribution of NEP activity among peripheral blood leukocytes.
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页码:116 / 125
页数:10
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