QUANTITATIVE-ANALYSIS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA IN ASYMPTOMATIC CARRIERS USING THE POLYMERASE CHAIN-REACTION

被引：47

作者：

OKA, S

URAYAMA, K

HIRABAYASHI, Y

OHNISHI, K

GOTO, H

MITAMURA, K

KIMURA, S

SHIMADA, K

机构：

[1] The Department of Infectious Diseases, the Institute of Medical Science, the University of Tokyo 4-6-1, Minato-ku, Tokyo, 108, Shirokanedai

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91721-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for detecting human immunodeficiency virus type 1 (HIV-1) provirus DNA in lymphocytes with improved sensitivity and reproducibility was developed using the polymerase chain reaction(PCR). Amplified HIV-1 DNA was hybridized with a 32P-labeled probe and quantitated with a beta-scanner after electrophoresis. A linear relationship was obtained between the common logarithms of the counts detected and the number of HIV-1 DNA copies applied to the PCR. Detectability was from 3 copies/105 lymphocytes, and linearity was maintained from 10 to 103 copies. HIV-1 DNA was detected in all 9 asymptomatic carriers tested (18 to 2,857 copies/105 CD4+ T lymphocytes). The viral burden was inversely related to the CD4+ lymphocyte count, suggesting that quantitation of provirus levels may serve as a predictor of progress in early HIV infection. © 1990.

引用

页码：1 / 8

页数：8

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