THE EFFECT OF OXYGEN RADICALS ON PROTEOLYSIS IN ISOLATED OAT CHLOROPLASTS

被引:22
作者
CASANO, LM
TRIPPI, VS
机构
[1] Laboratorio de Fisiologia Vegetal (IMBIV), Facultad de Ciencias Exactas, Fisicas y Naturales, Universidad National de Cordoba, (5000) Cordoba
关键词
ACTIVE OXYGEN; CHLOROPLASTS; PROTEOLYSIS;
D O I
10.1093/oxfordjournals.pcp.a078259
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
When chloroplasts isolated from oat leaves were incubated in the light, proteolysis and the activity of an acid endopeptidase increased. The increases were suppressed by 5 mM mannitol, an hydroxyl radical scavenger. The proteolysis and the endopeptidase also increased when chloroplasts were treated with Asc-Fe system, which can generate hydroxyl radical. In the polypeptides contained in chloroplasts, the hydrolysis of seven polypeptides was promoted by the treatment with Asc-Fe. Results suggest that oxygen radicals might mediate the light- and oxygen-induced proteolysis.
引用
收藏
页码:329 / 332
页数:4
相关论文
共 16 条
[1]  
BRADLEY R, 1991, PLANT PHYSL S, V96, P786
[2]  
CASANO LM, 1990, PLANT CELL PHYSIOL, V31, P377
[3]  
DALLING MJ, 1986, PLANT PROTEOLYTIC EN, V2, P125
[4]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9895
[5]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9914
[6]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P8227
[7]  
DAVIES KJA, 1988, FREE RADICAL BIO MED, V5, P216
[8]  
DORO GMD, 1987, PLANT CELL PHYSIOL, V28, P1389
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]  
LETO KJ, 1985, MOL BIOL PHOTOSYNTHE, P39