MULTIFUNCTIONAL ROLES OF A HISTIDINE RESIDUE IN HUMAN PANCREATIC ALPHA-AMYLASE

被引：30

作者：

ISHIKAWA, K ^{[1
]}

MATSUI, I ^{[1
]}

HONDA, K ^{[1
]}

NAKATANI, H ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,SAKYO KU,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91641-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functional roles of histidine residues at the active site in human pancreatic α -amylase were examined by protein engineering. Three histidine residues at 101, 201, and 299 were converted to asparagine residues, respectively. It was found that His201 played multi-functional roles concerning so many functions; substrate binding, control of optimum pH, change in substrate specificity, activation by chloride ion, and inhibition by a proteinaceous inhibitor. © 1992.

引用

页码：286 / 291

页数：6

共 26 条

[1]

[Anonymous], 1960, ENZYMES

[2] 3 DIMENSIONAL STRUCTURE OF PORCINE PANCREATIC ALPHA-AMYLASE AT 2.9 A RESOLUTION - ROLE OF CALCIUM IN STRUCTURE AND ACTIVITY [J].

BUISSON, G ;

DUEE, E ;

HASER, R ;

PAYAN, F .

EMBO JOURNAL, 1987, 6 (13) :3909-3916

[3] Alpha-amylase tertiary structures and their interactions with polysaccharides [J].

Buisson, G. ;

Duee, E. ;

Payan, F. ;

Haser, R. .

FOOD HYDROCOLLOIDS, 1987, 1 (5-6) :399-406

[4]

CANTER P, 1987, SCIENCE, V237, P394

[5] DETERMINATION OF REDUCING SUGAR WITH IMPROVED PRECISION [J].

DYGERT, S ;

LI, LH ;

FLORIDA, D ;

THOMA, JA .

ANALYTICAL BIOCHEMISTRY, 1965, 13 (03) :367-&

[6] NEW SUBSTRATE-SPECIFICITY OF MODIFIED PORCINE PANCREATIC ALPHA-AMYLASE [J].

ISHIKAWA, K ;

HIRATA, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 272 (02) :356-363

[7] THE PH-DEPENDENCE OF THE ACTION PATTERN IN PORCINE PANCREATIC ALPHA-AMYLASE-CATALYZED REACTION FOR MALTOOLIGOSACCHARIDE SUBSTRATES [J].

ISHIKAWA, K ;

MATSUI, I ;

HONDA, K ;

KOBAYASHI, S ;

NAKATANI, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 289 (01) :124-129

[8] SUBSTRATE-DEPENDENT SHIFT OF OPTIMUM PH IN PORCINE PANCREATIC ALPHA-AMYLASE-CATALYZED REACTIONS [J].

ISHIKAWA, K ;

MATSUI, I ;

HONDA, K ;

NAKATANI, H .

BIOCHEMISTRY, 1990, 29 (30) :7119-7123

[9] KINETIC-STUDY ON CHEMICAL MODIFICATION OF TAKA-AMYLASE-A .2. ETHOXYCARBONYLATION OF HISTIDINE-RESIDUES [J].

KITA, Y ;

SAKAGUCHI, S ;

NITTA, Y ;

WATANABE, T .

JOURNAL OF BIOCHEMISTRY, 1982, 92 (05) :1499-1504

[10] RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION [J].

KUNKEL, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :488-492

← 1 2 3 →