PRODUCTION OF AN ANTISERUM USING A FUSION PROTEIN PRODUCED BY A CDNA FOR RAT CHOLINE-ACETYLTRANSFERASE

被引：15

作者：

ICHIKAWA, T

OHSAKO, S

DEGUCHI, T

机构：

[1] TOKYO METROPOLITAN INST NEUROSCI, DEPT IMMUNOL, FUCHU, TOKYO 183, JAPAN

[2] TOKYO METROPOLITAN INST NEUROSCI, DEPT MOLEC NEUROBIOL, FUCHU, TOKYO 183, JAPAN

来源：

NEUROSCIENCE LETTERS | 1991年 / 131卷 / 02期

关键词：

CHOLINE ACETYLTRANSFERASE; FUSION PROTEIN; ANTISERUM; IMMUNOBLOT; IMMUNOHISTOCHEMISTRY; CHOLINERGIC NEURON;

D O I：

10.1016/0304-3940(91)90616-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A fusion protein produced by a cDNA for rat choline acetyltransferase (ChAT) inserted into a translation vector was used for immunization of rabbits. An antiserum was obtained that could recognize a single protein band in immunoblot analysis of a partially purified enzyme preparation of the rat brain. The antiserum revealed ChAT immunoreactivity in the motoneurons and terminal-like structures in the neuropil of the ventral horn in cryostat sections of the cervical spinal cord of the rat. This antiserum may be of particular use to study the development of the cholinergic neuron.

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页码：213 / 216

页数：4

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