DISCORDANT REGULATION OF HUMAN TYPE-I COLLAGEN GENES BY PROSTAGLANDIN-E2

被引：21

作者：

FINE, A ^{[1
]}

MATSUI, R ^{[1
]}

ZHAN, X ^{[1
]}

POLIKS, CF ^{[1
]}

SMITH, BD ^{[1
]}

GOLDSTEIN, RH ^{[1
]}

机构：

[1] VET ADM MED CTR,BOSTON,MA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1135卷 / 01期

关键词：

PROSTAGLANDIN-E2; CYCLIC AMP; COLLAGEN;

D O I：

10.1016/0167-4889(92)90167-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We examined the expression of type I collagen genes in prostaglandin E2 (PGE2)-treated human lung fibroblast cultures. Addition of PGE2 to fibroblast cultures inhibited alpha-1(I) mRNA levels by approx. 25% after 6 h and 60% after 24 h. Further studies showed that dibutyryl cAMP did not inhibit alpha-1(I) mRNA levels and that cycloheximide blocked the inhibitory effect of PGE2. In contrast, PGE2 treatment with or without cycloheximide did not affect alpha-2(I) mRNA levels. Moreover, in vitro translation of RNA derived from untreated and PGE2-treated cells yielded similar amounts of alpha-2(I) collagen peptides. Taken together, these results suggest that PGE2 induces a protein which inhibits alpha-1(I) transcription through distinct regulatory elements not under the control of cAMP and provide further evidence that the type I collagen genes can be discordantly regulated.

引用

页码：67 / 72

页数：6

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