Differentiation of field bean heterochromatin by in situ hybridization with a repeated Fok I sequence

被引:51
作者
Fuchs, J. [1 ]
Pich, U. [1 ]
Meister, A. [1 ]
Schubert, I. [1 ]
机构
[1] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany
关键词
chromosome banding; FISH; flow sorting; Fok I sequence repeats; heterochromatin; Vicia faba;
D O I
10.1007/BF01539450
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chromosomes of a field bean line with a reconstructed karyotype (ACB) were hybridized in situ with biotinylated probes of a repetitive Fok I sequence, of DOP-PCR (degenerate oligonucleotide primed polymerase chain reaction) amplified DNA from a chromosome that does not contain this sequence, and with probes containing dispersed repetitive sequences. The results were compared with Giemsa banding, DNA late replication and Fok I in situ digestion patterns. This allowed further differentiation between the chromatin types of this species. Centromeric and NOR-associated heterochromatin as well as euchromatin were shown to be free of Fok I sequence repeats. Among the interstitial late replicating Giemsa bands, subdivided into 'marker' and 'additional' bands, most of the marker bands located at mid-arm positions were composed mainly or exclusively of tandemly arranged Fok I repeats. Some of the marker bands and nearly all of the additional bands located in the vicinity of centromeres were free of Fold sequence repeats, of Fok I recognition sites, and possibly also of dispersed repetitive sequences. They are probably composed of specific, not yet defined, repetitive sequences.
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页码:25 / 28
页数:4
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