PROTON ACTIVITIES FOR 3-STATES OF CYTOCHROME-P-450CAM

被引：18

作者：

DOLPHIN, D

JAMES, BR

WELBORN, HC

机构：

[1] Department of Chemistry, University of British Columbia, Vancouver

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)92064-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in proton concentration during the binding of dioxygen, carbon monoxide, and for the exchange of dioxygen by carbon monoxide, at ferrous-cytochrome P-450cam were measured by direct titration. Insufficient proton release was observed to support protonation-deprotonation of an axial cysteinyl sulfur donor as a mechanism for generation of hyper spectra in only the carbonylated ferrous state. Measurement of the P 1 2 value for CO binding as a function of pH (the carbon monoxide Bohr effect) confirms the direct titration data. © 1979.

引用

页码：415 / 421

页数：7

共 20 条

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