REDUCTANT FOR GLUTAMATE SYNTHASE IS GENERATED BY THE OXIDATIVE PENTOSE-PHOSPHATE PATHWAY IN NONPHOTOSYNTHETIC ROOT PLASTIDS

被引：131

作者：

BOWSHER, CG ^{[1
]}

BOULTON, EL ^{[1
]}

ROSE, JKC ^{[1
]}

NAYAGAM, S ^{[1
]}

EMES, MJ ^{[1
]}

机构：

[1] UNIV MANCHESTER, DEPT CELL & STRUCT BIOL, PLANT METAB RES UNIT, WILLIAMSON BLDG, MANCHESTER M13 9PL, LANCS, ENGLAND

来源：

PLANT JOURNAL | 1992年 / 2卷 / 06期

关键词：

D O I：

10.1111/j.1365-313X.1992.00893.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Purified pea root plastids were supplied with glutamine, 2-oxoglutarate and phosphorylated sugars. Formation of glutamate was linear for 75 min and dependent upon the intactness of the organelle. Glucose-6-phosphate and ribose-5-phosphate were the most effective substrates in supporting glutamate synthesis. Flux through the oxidative pentose phosphate pathway during glutamate synthesis in purified plastids was followed by monitoring the release of (CO2)-C-14 from [1-C-14]glucose-6-phosphate. (CO2)-C-14 evolution from C-1 was dependent upon the presence of both glutamine and 2-oxoglutarate and could be inhibited by the application of azaserine. The data are discussed in view of the role of the oxidative pentose phosphate pathway in non-photosynthetic plastids.

引用

页码：893 / 898

页数：6

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