PHARMACOLOGICAL STUDIES ON HELIX NEURON OCTOPAMINE RECEPTORS

被引:32
作者
BATTA, S
WALKER, RJ
WOODRUFF, GN
机构
[1] School of Biochemical and Physiological Sciences, University of Southampton, Southampton
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1979年 / 64卷 / 01期
关键词
D O I
10.1016/0306-4492(79)90027-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. Intracellular recordings have been made from identified neurons in the suboesophageal ganglionic mass of the snail, Helix aspersa. Four cells were found which were inhibited by octopamine and one cell which was excited. The responses of these cells to dopamine, noradrenaline, 5-hydroxytryptamine, glutamic acid and acetylcholine were determined. 2. A structure-activity study was performed on two cells, cell F89 which was inhibited by octopamine, dopamine and noradrenaline and cell E26 which was excited by octopamine but inhibited by both dopamine and noradrenaline. The results suggest evidence for a specific octopamine receptor. 3. A series of compounds were tested for potential blocking activity against octopamine, dopamine and noradrenaline. Phentolamine, 2 × 10-7 M, blocked the action of octopamine and noradrenaline on cell 89 without affecting that of dopamine. On cell E26 phentolamine, 2 × 10-7 M, blocked the excitatory action of octopamine without any effect on dopamine and noradrenaline inhibition. Fluphenazine, 2 × 10-7 M, blocked the action of dopamine and noradrenaline without affecting the octopamine response of either cell. 4. The present data present further evidence for a specific octopamine receptor on Helix neurons. The requirements for optimal activity are: a hydroxyl group on the para position of the benzene ring, an ethylamine side chain with a hydroxyl group on the beta carbon, substitution of no more than one methyl group on the terminal nitrogen. © 1979.
引用
收藏
页码:43 / 51
页数:9
相关论文
共 34 条
  • [1] OCTOPAMINE-SENSITIVE ADENYLATE-CYCLASE IN CENTRAL NERVOUS-SYSTEM OF LIMULUS-POLYPHEMUS
    ATKINSON, MM
    HERMAN, WS
    SHEPPARD, JR
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1977, 58 (02): : 107 - 110
  • [2] OCTOPAMINE IN LOBSTER NERVOUS-SYSTEM
    BARKER, DL
    MOLINOFF, PB
    KRAVITZ, EA
    [J]. NATURE-NEW BIOLOGY, 1972, 236 (63): : 61 - &
  • [3] SYNTHESIS OF DOPAMINE AND OCTOPAMINE IN THE CRUSTACEAN STOMATOGASTRIC NERVOUS-SYSTEM
    BARKER, DL
    KUSHNER, PD
    HOOPER, NK
    [J]. BRAIN RESEARCH, 1979, 161 (01) : 99 - 113
  • [4] BATTA S, 1977, J PHYSIOLOGY, V270, P63
  • [5] NORMAL OCCURRENCE OF OCTOPAMINE IN CENTRAL NERVOUS-SYSTEM OF RAT
    BUCK, SH
    MURPHY, RC
    MOLINOFF, PB
    [J]. BRAIN RESEARCH, 1977, 122 (02) : 281 - 297
  • [6] CARLSON AD, 1968, J EXP BIOL, V49, P195
  • [7] CARLSON AD, 1972, J EXP BIOL, V57, P737
  • [8] OCTOPAMINE RECEPTORS ON APLYSIA NEURONS MEDIATE HYPERPOLARIZATION BY INCREASING MEMBRANE CONDUCTANCE
    CARPENTER, DO
    GAUBATZ, GL
    [J]. NATURE, 1974, 252 (5483) : 483 - 485
  • [9] DIFFERENTIAL BLOCKADE OF OCTOPAMINE AND DOPAMINE RECEPTORS BY ANALOGS OF CLOZAPINE AND METOCLOPRAMIDE
    DOUGAN, DFH
    WADE, DN
    [J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1978, 5 (04) : 341 - 349
  • [10] DOUGAN DFH, 1977, CLIN EXP PHARMACOL P, V4, P219