THE LY-1 GENE-EXPRESSION IN MURINE HYBRIDOMAS PRODUCING AUTOANTIBODIES

被引：11

作者：

MAYER, R ^{[1
]}

ZAGHOUANI, H ^{[1
]}

USUBA, O ^{[1
]}

BONA, C ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT MICROBIOL,ANNENBERG 16-60,1 GUSTAVE LEVY PL,NEW YORK,NY 10029

来源：

AUTOIMMUNITY | 1990年 / 6卷 / 04期

关键词：

Autoantibodies; Hybridomas; Ly-l; Motheaten; NZB;

D O I：

10.3109/08916939008998421

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Studies presented here demonstrate the expression of the Ly-l gene and the detection of the Ly-l cytodifferentiation antigen in murine hybridomas producing autoantibodies. We examined the transcription of the Ly-I gene in thymocytes and 140 hybridomas producing autoantibodies of various specificities which were obtained from normal and autoimmune disease prone mouse strains. As previously demonstrated thymocytes stain brightly for Ly-l by immunofluorescence and express Ly-l transcripts. In our panel of hybridomas producing autoantibodies Ly-l transcripts were detected in 31 (45% out of 69 NZB hybridomas and 7 (88% out of 8 viable motheaten hybridomas. S1 nuclease protection experiments showed that Ly-l transcripts detected in thymocytes and B cells are the product of the same gene. The B cell transcripts are functional since immunofluorescence and Western data presented here detected the Ly-I protein in hybridomas cells which were found to transcribe the Ly-l gene. Interestingly a polymorphic transcription of the Ly-l gene was observed in B cells and B cell hybridomas as compared to thymocytes. Our results obtained in the hybridoma system firmly establish a major contribution of the Ly-l B cell subset to the production of DNA specific autoantibodies and a smaller contribution to the production of rheumatoid factors and "natural" multispecific autoantibodies. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：293 / 305

页数：13

共 20 条

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