EXPRESSION OF THE BROWN FAT MITOCHONDRIA UNCOUPLING PROTEIN IN XENOPUS OOCYTES AND IMPORT INTO MITOCHONDRIAL-MEMBRANE

被引：15

作者：

KLAUS, S

CASTEILLA, L

BOUILLAUD, F

RAIMBAULT, S

RICQUIER, D

机构：

[1] Centre National de la Recherche Scientifique, Centre de Recherches sur la Nutrition, F-92190 Meudon-Bellevue, 9, rue Jules Hetzel

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92094-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non shivering thermogenesis of brown adipose tissue is due to the uncoupling protein (UCP), located in the inner mitochondrial membrane, which functions as a proton translocator and can thus uncouple mitochondrial respiration. We describe here the expression of UCP in Xenopus laevis oocytes after injection of UCP mRNA, which was transcribed in vitro. UCP seems to be correctly transported into mitochondria and integrated into the membrane, but we were not able to establish definitely the functionality of this UCP. We conclude that this expression system could be suitable for the study of the mitochondrial import mechanism but not for the examination of physiological properties of UCP. © 1990.

引用

页码：784 / 789

页数：6

共 14 条

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