PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR LIPASE OF GALACTOMYCES-GEOTRICHUM

被引：19

作者：

PHILLIPS, A

PRETORIUS, GHJ

机构：

[1] UNIV ORANGE FREE STATE, DEPT MICROBIOL & BIOCHEM, POB 339, BLOEMFONTEIN 9300, SOUTH AFRICA

[2] UNIV ORANGE FREE STATE, SASOL BIOTECHNOL CTR, BLOEMFONTEIN 9300, SOUTH AFRICA

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 11期

关键词：

D O I：

10.1007/BF01026769

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Galactomyces geotrichum secretes an extracellular lipase with a relative molecular weight of 57 +/- 2 kDa in the unglycosylated state and 62 +/- 2 kDa when glycosylated. The enzyme has optimal activity at pH 7.75 and 30-degrees-C and has a pI of 5.2. The specificity of the lipase is similar to lipase A of Geotrichum candidum, but the amino acid composition of the lipases are different.

引用

页码：833 / 838

页数：6

共 17 条

[1]

BAILLARGEON MW, 1989, APPL MICROBIOL BIOT, V30, P92

[2] PURIFICATION AND SPECIFICITY OF LIPASES FROM GEOTRICHUM-CANDIDUM [J].