3-DIMENSIONAL STRUCTURE OF THE SURFACE-LAYER OF WOLINELLA-RECTA

被引：10

作者：

DOKLAND, T

OLSEN, I

FARRANTS, G

JOHANSEN, BV

机构：

[1] Emu for Biological Sciences, University of Oslo

[2] Inst. of Microbiology, Dental Faculty, University of Oslo

[3] Laboratory for Experimental Pathology and Image Analysis, Norwegian Radium Hospital, Inst. for Cancer Research

[4] Dept. of Methodology, National Inst. of Public Health, Oslo

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 03期

关键词：

3‐dimensional reconstruction; surface layer; Wolinella recta;

D O I：

10.1111/j.1399-302X.1990.tb00415.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The three‐dimensional structure of the crystalline surface layer (S‐layer) of Wolinella recta ATCC 33238T, a gram‐negative, anaerobic periodontopathogen, was determined to 3.8 nm resolution by electron microscopy and digital image processing. The S‐layer protein is closely associated with the outer bacterial membrane, and shows p6 symmetry with lattice spacing and thickness of 21 nm and 15 nm, respectively. The funnel‐shaped subunits consist of 6 heavy domains located round a common base at the sixfold axis, and communicate with the adjacent subunits through a lighter domain at the threefold axis (M6C3 arrangement). Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：162 / 165

页数：4

共 20 条

[1] Amos LA, Henderson R, Unwin PNT, Three‐dimensional structure determination by electron microscopy of two dimensional crystals, Progress in Biophysics and Molecular Biology, 39, pp. 183-231, (1982)
[2] Baumeisler W, Engelhardt H, Three‐dimensional structure of bacterial surface layers, Electron microscopy of proteins, 6, pp. 109-154, (1987)
[3] Baumeister W, Barth M, Hegerl R, Guckenberger R, Hahn M, Saxton WO, Three‐dimensional structure of the regular surface layer (HPI‐layer) of Deinococcus radiodurans, J Mol Biol, 187, pp. 241-253, (1986)
[4] Dickson MR, Downing KH, Wu WH, Glucser RM, Three‐dimensional structure of the surface layer protein of Aquaspirillum serpens VHA, J Bacteriol, 167, pp. 1025-1034, (1986)
[5] Dokland T, (1988)
[6] Dzink JL, Tanner ACR, Haffajee AD, Socransky SS, Gram negative species associated with active periodontal lesions, J Clin Periodontol, 12, pp. 648-659, (1985)
[7] Every D, Skerman TM, Surface structure of Bacteroides nodosus in relation to virulence and immunoproleclion in sheep, J Gen Microbiol, 129, pp. 225-234, (1983)
[8] Gillespie J, Holl SC, Growth studies of Wolinella recta, a gram‐negative period ontopathogen, Oral Microbiol Immunol, 2, pp. 105-111, (1987)
[9] Haapasalo M, Bacteroides buccae and related taxa in necrotic root canal infections, J Clin Microbiol, 24, pp. 940-944, (1986)
[10] Henderson R, Baldwin JM, Downing KH, Lepault J, Zemlin F, Structure of purple membrane from Halobacterium halobium: Recording measurement and evaluation of electron micrographs at 3.5 A resolution, Ultramicroscopy, 19, pp. 147-178, (1986)

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