SPECIFIC, SENSITIVE METHOD FOR THE DETERMINATION OF HYALURONATE

被引：41

作者：

JOURDIAN, GW

WOLFMAN, M

SARBER, R

DISTLER, J

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48109

[2] UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI 48109

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90609-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The β-Δ4,5-eneglucopyranuronic acid residues contained at the nonreducing termini of the resulting oligosaccharides were oxidized with periodic acid to yield, among other products, formyl pyruvic acid. The latter compound reacted with thiobarbituric acid to yield a chromophore with an absorption maximum at 549 nm. Optimal conditions for quantitative assay of hyaluronate are described. © 1979.

引用

页码：474 / 480

页数：7

共 22 条

[1] METHODS FOR QUANTITATIVE ESTIMATION OF N-ACETYLNEURAMINIC ACID AND THEIR APPLICATION TO HYDROLYSATES OF SIALOMUCOIDS [J].