PARTICULAR CHARACTERISTICS OF THE ANTI-HUMAN CYTOMEGALOVIRUS ACTIVITY OF (S)-1-(3-HYDROXY-2-PHOSPHONYLMETHOXYPROPYL)CYTOSINE (HPMPC) INVITRO

被引：59

作者：

NEYTS, J ^{[1
]}

SNOECK, R ^{[1
]}

BALZARINI, J ^{[1
]}

DECLERCQ, E ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN, REGA INST MED RES, MINDERBROEDERSSTR 10, B-3000 LOUVAIN, BELGIUM

来源：

ANTIVIRAL RESEARCH | 1991年 / 16卷 / 01期

关键词：

CYTOMEGALOVIRUS; HPMPC; DNA INTEGRITY;

D O I：

10.1016/0166-3542(91)90057-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The acyclic nucleoside phosphonate analogue (S)-1-[3-hydroxy-2-phosphonylmethoxypropyl]cytosine (HPMPC) is a potent and selective inhibitor of human cytomegalovirus (HCMV) replication and DNA synthesis. Unlike 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG), HPMPC inhibits HCMV replication in cell cultures which have been treated with the compound before infection. Upon short-pulse treatment of HCMV-infected cells with HPMPC, a long-lasting antiviral response is obtained. The antiviral activity of HPMPC, unlike the antiviral activity of other cytosine derivatives (i.e. Ara-C, FIAC), is not readily reversed by 2'-deoxycytidine or cytidine. Neutral and alkaline sucrose gradient analysis of HCMV DNA isolated from HPMPC-treated HCMV-infected cell cultures revealed that HPMPC does not cause (detectable) single- or double-strand breakage of HCMV DNA.

引用

页码：41 / 52

页数：12

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