ROLE OF SINGLET OXYGEN IN PHOTO-OXIDATION OF EXCITABLE CELL-MEMBRANES

被引：49

作者：

POOLER, JP

VALENZENO, DP

机构：

[1] Department of Physiology, Emory University, Atlanta, Georgia

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 30卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1979.tb07183.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— Giant nerve axons from lobsters were photooxidized by visible light from a Xe arc using eosin Y, rose bengal and methylene blue as sensitizers. The degree of modification was assayed as the rate constant for block of sodium channels during illumination. Protection or enhancement of modification was tested for several reagents known to interact with singlet oxygen. Deuterium oxide enhanced the modification up to 57% while sodium azide protected up to 60%. β ‐Carotene, triethylamine and DABCO exerted no effect. From a consideration of the probable low polarity environment of the sensitization sites and diffusional restrictions on reagents it is concluded that intramembranously created singlet oxygen plays a significant role in membrane modification. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：581 / 584

页数：4

共 19 条

[1] PHOTODYNAMIC EFFECTS OF PROTOPORPHYRIN ON HUMAN ERYTHROCYTES - NATURE OF CROSS-LINKING OF MEMBRANE PROTEINS [J].