PROTECTIVE ACTION OF A NEW BENZOFURAN DERIVATIVE ON LIPID-PEROXIDATION AND SULFHYDRYL-GROUPS OXIDATION

The antioxidant properties of a novel water-soluble antioxidant of the benzofuran family (5-hydroxy-4, 6, 7-trimethyl-2, 3-dihydrobenzofuran-2-acetic acid, BFA) were studied. In rat liver mitochondria BFA increases the lag-time and decreases the extent of lipid peroxidation induced by ascorbate/Fe2+; an IC50 value of about 12 μm was observed. In rat liver microsomes it inhibits the lipid peroxidation induced both by NADPH/Fe2+/ADP (iron-dependent) and by cumene hydroperoxide (iron-independent), showing IC50 values of 25 and 30 μm respectively. The antioxidant efficiency of BFA is slightly higher than that of the congener compound Trolox C. BFA is also able to inhibit the oxidation of protein sulphydryl groups consequent to microsomal lipid peroxidation induced by NADPH/Fe2+/ADP. The antioxidant properties of BFA are discussed considering its hydrophilic character and pharmacological features. © 1991 The Italian Pharmacological Society.