CHROMATOGRAPHIC AND SPECTROSCOPIC ANALYSIS OF GLOBOTRIAOSYL CERAMIDE FROM BOVINE SPERMATOZOA

被引：3

作者：

ALVAREZ, JG

STOREY, BT

HEMLING, ML

GROB, RL

机构：

[1] UNIV PENN, SCH MED, DEPT PHYSIOL, PHILADELPHIA, PA 19104 USA

[2] UNIV PENN, SCH MED, DEPT OBSTET & GYNECOL, PHILADELPHIA, PA 19104 USA

[3] VILLANOVA UNIV, DEPT CHEM, VILLANOVA, PA 19085 USA

[4] SMITHKLINE BEECHAM LABS, KING OF PRUSSIA, PA USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/10826079208018314

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The isolation and characterization of globotriaosyl ceramide from bovine spermatozoa is presented. Analysis of Gb3 by high-resolution proton nuclear magnetic resonance (H-1-NMR), fast atom bombardment mass spectrometry (FAB-MS), mobility on HPTLC, and HpTLC-immunostaining; GLC/MS analysis of the partially methylated alditol acetates of the sugar units, and GLC analysis of the sphingoid bases and fatty acids present in the ceramide moiety, resulted in the characterization of this major glycosphingolipid as Gal(alpha-1-4) Gal(beta-1-4)Glc(beta-1-1)Cer.

引用

页码：1621 / 1638

页数：18

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