TETRAHYDRO-6-BIOPTERIN IS ASSOCIATED WITH TETRAHYDRO-7-BIOPTERIN IN PRIMARY MURINE MAST-CELLS

被引：16

作者：

ZIEGLER, I ^{[1
]}

HULTNER, L ^{[1
]}

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH,INST EXPTL HAMATOL,W-8000 MUNICH 70,GERMANY

来源：

FEBS LETTERS | 1992年 / 307卷 / 02期

关键词：

MAST CELL; TETRAHYDRO-6-BIOPTERIN; TETRAHYDRO-7-BIOPTERIN; 6-BIOPTERIN; 7-BIOPTERIN; TRYPTOPHAN; 5-MONO-OXYGENASE;

D O I：

10.1016/0014-5793(92)80755-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine bone marrow-derived mast cells proliferate in response to interleukin 3. In addition to 6-biopterin, 7-biopterin was identified in these cells by HPLC analysis of iodine oxidized extracts and by alkaline permanganate oxidation to the 6- and 7-carboxylic acids. 7-Biopterin comprised 31.9 (+/- 7.7)% of the total biopterin. It was absent in cells which were grown with of L-p-chlorophenylalanine, an inhibitor of tryptophan 5-mono-oxygenase. Both 6- and 7-biopterin were present in thc cell as their tetrahydro forms. From these data we conclude that 7-biopterin, in contrast to e.g. brain tissue, regularly occurs as a normal metabolite in primary mast cells and that it is generated during hydroxylation of tryptophan.

引用

页码：147 / 150

页数：4

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