EXPRESSION OF BROME MOSAIC VIRUS-ENCODED REPLICASE GENES IN TRANSGENIC TOBACCO PLANTS

被引：26

作者：

MORI, M

MISE, K

OKUNO, T

FURUSAWA, I

机构：

[1] Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Sakyo-ku

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-1-169

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We introduced replicase genes of brome mosaic virus (BMV) to Nicotiana tabacum cv. Petit Habana (SRI) using two different types of transformation vectors containing cDNAs of BMV RNA 1 and RNA 2. One type (V type) contains cDNA from which complete viral RNAs are transcribed. These RNAs can function as templates for viral replicase. The other type (M type) contains cDNA from which viral RNAs without their 3' non-coding regions are transcribed; these RNAs can only function as mRNA. Viral replicase expressed from the integrated cDNAs in both V and M type transgenic plants can complement an infection by BMV RNA 3.

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页码：169 / 172

页数：4

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