ASSAY OF N-ACETYL-BETA-D-GLUCOSAMINIDASE IN URINE FROM NEONATES - COMPARISON OF 2 NEW COLORIMETRIC METHODS USING MNP-GLCNAC AND VRA-GLCNAC AS SUBSTRATES

被引：9

作者：

POCSI, I

CSATHY, L

OLAH, VA

PRICE, RG

机构：

[1] CTY HOSP,DEPT PEDIAT,H-4043 DEBRECEN,HUNGARY

[2] KINGS COLL LONDON,DIV BIOMOLEC SCI,BIOCHEM SECT,LONDON W8 7AH,ENGLAND

[3] DEBRECEN UNIV MED,SCH MED,DEPT PEDIAT,H-4012 DEBRECEN,HUNGARY

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1992年 / 29卷

关键词：

NAG INDEXES; SENSITIVITY; IMPRECISION;

D O I：

10.1177/000456329202900307

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The NAG activity present in urine from newborn babies was assayed using two colorimetric procedures with either MNP-GlcNAc or VRA-GlcNAc as substrate and compared with data obtained with the well established PNP-GlcNAc procedure. Both new assays were easy to perform and reproducible. The MNP-GlcNAc method has the advantage that it is now available as a kit; however, the VRA-GlcNAc procedure is more sensitive. NAG activity, creatinine concentration and NAG-index values were determined in normal neonates and within-run imprecision calculated. Excellent correlations were found between MNP-GlcNAc-ase and VRA-GlcNAc-ase indices (r = 0.984) and between PNP-GlcNAc-ase and VRA-GlcNAc-ase indices (r = 0.952). When low molecular weight urinary components were removed by gel filtration no significant change in VRA-GlcNAc-ase activity was observed.

引用

页码：292 / 295

页数：4

共 18 条

[1]

BONSNES RW, 1945, J BIOL CHEM, V158, P581

[2]

BOUDIOU MT, 1985, CLIN CHIM ACTA, V149, P67

[3] THE EFFECT OF POLYCYTHEMIA AND HYPOXIA ON URINARY N-ACETYL-BETA-D-GLUCOSAMINIDASE ACTIVITY IN NEWBORNS [J].

CSATHY, L ;

POCSI, I ;

KISS, L ;

BALLA, G ;

MODI, N ;

PRICE, RG .

CLINICA CHIMICA ACTA, 1990, 195 (1-2) :77-85

[4] URINARY-EXCRETION OF N-ACETYL-GLUCOSAMINIDASE AND BETA-2-MICROGLOBULIN AS EARLY MARKERS OF GENTAMICIN-NEPHROTOXICITY IN NEONATES [J].

GOUYON, JB ;

AUJARD, Y ;

ABISROR, A ;

LAUDIGNON, N ;

DATHIS, P ;

JACQZ, E ;

BIOU, D ;

DEMELIER, JF ;

MATHIEU, H .

DEVELOPMENTAL PHARMACOLOGY AND THERAPEUTICS, 1987, 10 (02) :145-152

[5]

HORAK E, 1981, CLIN CHEM, V27, P1180

[6]

JOHNSON CE, 1990, PEDIATRICS, V86, P211

[7]

LANGHENDRIES JP, 1989, BIOL NEONATE, V56, P76

[8] RAPID COLORIMETRIC ASSAY OF BETA-GALACTOSIDASE AND N-ACETYL-BETA-GLUCOSAMINIDASE IN HUMAN URINE [J].

MARUHN, D .

CLINICA CHIMICA ACTA, 1976, 73 (03) :453-461

[9]

OKADA S, 1971, NEW ENGL J MED, V285, P409

[10]

POCSI I, 1990, CLIN CHEM, V36, P1884

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