LIMITED PROTEOLYSIS AND SITE-DIRECTED MUTAGENESIS OF THE NF-KAPPA-B P50 DNA-BINDING SUBUNIT

被引：46

作者：

SODEOKA, M

LARSON, CJ

CHEN, L

LANE, WS

VERDINE, GL

机构：

[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138

[2] HARVARD UNIV,MICROCHEM FACIL,CAMBRIDGE,MA 02138

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 06期

关键词：

D O I：

10.1016/S0960-894X(00)80294-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Tryptic digestion was used to obtain an active 41 kDa fragment of NF-kappaB. The major proteolysis products were comprised of residues 2-364 (p50(2-364)), 2-362 (p50(2-362)) and 2-366 (p50(2-366)). By site-directed truncation mutagenesis, overproducers for p50(2-364) and p50(2-366) were constructed. These vectors direct high level expression of homogeneous, active protein.

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页码：1095 / 1100

页数：6

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