A STUDY OF URICASE BIOSENSOR BASED ON A GLASSY-CARBON ELECTRODE MODIFIED WITH NAFION AND METHYL VIOLOGEN

被引：16

作者：

JIN, LT

YE, JS

TONG, W

FANG, YZ

机构：

[1] Department of Chemistry, East China Normal University, Shanghai, 200062

来源：

MIKROCHIMICA ACTA | 1993年 / 112卷 / 1-4期

关键词：

URICASE; BIOSENSOR; NAFION-MV MODIFIED ELECTRODE;

D O I：

10.1007/BF01243323

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A uricase biosensor was constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize uricase on a glassy carbon electrode modified with Nafion and methyl viologen (MV). A linear response of uric acid in serum was observed in a range of 1.0 . 10(-3) to 5.0 . 10(-6) mol/l and the response time is 25 s. This biosensor has the advantage of high sensitivity, fast response as compared with previous sensors and low interferences.

引用

页码：71 / 75

页数：5

共 7 条

[1] IMMOBILIZED ENZYMES - APPLICATION OF IMMOBILIZED URATE OXIDASE TO CHEMICAL-ANALYSIS OF URIC-ACID IN BIOLOGICAL-FLUIDS
DRITSCHILO, W
WEIBEL, MK
[J]. BIOCHEMICAL MEDICINE, 1974, 11 (03): : 242 - 249
[2] GUILBAULT G, 1984, ANAL USES IMMOBILIZE
[3] DETERMINATION OF DISSOLVED-OXYGEN BY CATALYTIC REDUCTION ON NAFION-METHYL VIOLOGEN CHEMICALLY MODIFIED ELECTRODE
JIN, LT
PING, J
YE, JN
FANG, YZ
[J]. TALANTA, 1992, 39 (02) : 145 - 147
[4] JIN LT, 1991, J E CHINA NORMAL SEP, P50
[5] URIC-ACID SENSORS BASED ON THE USE OF A CARBON-DIOXIDE ELECTRODE AND ACTIVATION OF URICASE MEMBRANES
KAWASHIMA, T
ARIMA, A
HATAKEYAMA, N
TOMINAGA, N
ANDO, M
[J]. NIPPON KAGAKU KAISHI, 1980, (10) : 1542 - 1548
[6] ENZYME ELECTRODE SENSING OXYGEN FOR URIC-ACID IN SERUM AND URINE
NANJO, M
GUILBAULT, GG
[J]. ANALYTICAL CHEMISTRY, 1974, 46 (12) : 1769 - 1772
[7] 1979, CLIN BIOCH ANAL

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