SEQUENCE OF THE DNA LIGASE-ENCODING GENE FROM THERMUS-SCOTODUCTUS AND CONSERVED MOTIFS IN DNA LIGASES

被引：18

作者：

JONSSON, ZO ^{[1
]}

THORBJARNARDOTTIR, SH ^{[1
]}

EGGERTSSON, G ^{[1
]}

PALSDOTTIR, A ^{[1
]}

机构：

[1] UNIV ICELAND,INST BIOL,GENET MOLEC LAB,IS-108 REYKJAVIK,ICELAND

来源：

GENE | 1994年 / 151卷 / 1-2期

关键词：

DNA REPLICATION; NAD(+)-DEPENDENT DNA LIGASE; RECOMBINANT DNA; THERMOPHILIC BACTERIA; THERMOSTABLE ENZYME;

D O I：

10.1016/0378-1119(94)90652-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

By dideoxynucleotide sequencing of a genomic clone, we have determined the complete nucleotide sequence of the gene encoding NAD(+)-dependent DNA ligase (EC 6.5.1.2) of the thermophilic bacterium Thermus scotoductus. The gene encodes a 674-amino-acid thermostable enzyme highly similar to other bacterial DNA ligases and to parts of the deduced gene product of Escherichia coli ORF f562, 5' to the spoR gene encoding 5' guanosyl kinase.

引用

页码：177 / 180

页数：4

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