APPLICATION OF UREASE CONDUCTOMETRIC BIOSENSOR FOR HEAVY-METAL ION DETERMINATION

被引：90

作者：

ZHYLYAK, GA ^{[1
]}

DZYADEVICH, SV ^{[1
]}

KORPAN, YI ^{[1
]}

SOLDATKIN, AP ^{[1
]}

ELSKAYA, AV ^{[1
]}

机构：

[1] SHEVCHENKO KIEV UNIV,SECTOR BIOELECTR,KIEV 252001,UKRAINE

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1995年 / 24卷 / 1-3期

关键词：

BIOSENSORS; CONDUCTOMETRY; THIN-FILM INTERDIGITATED ELECTRODES; UREASE IMMOBILIZATION; HEAVY-METAL ION DETERMINATION;

D O I：

10.1016/0925-4005(95)85031-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Urease conductometric biosensors consisting of interdigitated gold electrodes and enzyme membranes formed on their sensitive parts have been used for a quantitative estimation of general water pollution with heavy-metal ions. The measurements of the urease residual activity have been carried out in Tris-HNO3 buffer after preincubation in model metal-salt solution. The detection limits, depending on preincubation time and dynamic ranges, have been determined in model solutions of heavy-metal ions. The sequence of metals ions relative to their toxicity toward urease is: Hg2+ > Cu-2+ > Cd?(2+ > Co2+ > Pb2+ > Sr2+ The conditions for practical applications of the, biosensors have been investigated and critically evaluated for optimization. Urease reactivation by EDTA after inhibition by heavy-metal ions has been demonstrated. The performance characteristics of the conductometric biosensor are discussed.

引用

页码：145 / 148

页数：4

共 10 条

[1] DIXON M, 1979, ENZYMES, V1
[2] DETECTION OF HEAVY-METAL SALTS WITH BIOSENSORS BUILT WITH AN OXYGEN-ELECTRODE COUPLED TO VARIOUS IMMOBILIZED OXIDASES AND DEHYDROGENASES
GAYET, JC
HAOUZ, A
GELOSOMEYER, A
BURSTEIN, C
[J]. BIOSENSORS & BIOELECTRONICS, 1993, 8 (3-4) : 177 - 183
[3] HIROMITSU S, 1991, SENSORS MATER, V2, P217
[4] KRAJEWSKA B, 1991, J CHEM TECHNOL BIOT, V52, P157
[5] MACGREGOR JT, 1974, PROTEIN METAL INTERA, P463
[6] THIN-FILM CONDUCTOMETRIC BIOSENSORS FOR GLUCOSE AND UREA DETERMINATION
SHULGA, AA
SOLDATKIN, AP
ELSKAYA, AV
DZYADEVICH, SV
PATSKOVSKY, SV
STRIKHA, VI
[J]. BIOSENSORS & BIOELECTRONICS, 1994, 9 (03) : 217 - 223
[7] SOLDATKIN A, 1993, Patent No. 9305941
[8] TRANMINH C, 1985, ION SEL ELECTRODE R, V7, P41
[9] WEBB JL, 1966, ENZYMES METABOL INH, V2, P729
[10] WEBB JL, 1966, ENZYMES METABOL INH, V2, P635

← 1 →