STOKES RADIUS OF 57CO-LABELED VITAMIN B12-TRANSCOBALAMIN I AND 2 DETERMINED BY SEPHADEX GEL FILTRATION

被引：24

作者：

HOM, BL

机构：

[1] Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 175卷 / 01期

关键词：

D O I：

10.1016/0005-2795(69)90140-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Stokes radii of the two principal vitamin B12-binding proteins of human serum have been estimated by gel filtration on Sephadex G-75 and G-200. Mean values of 51.3 Å for [57Co]B12-transcobalamin I and 25.5 Å for [57Co]B12-transcobalamin II were obtained. © 1969.

引用

收藏

页码：20 / &

相关论文

共 10 条

[1] MOLECULAR EXCLUSION + RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY [J].

ACKERS, GK .

BIOCHEMISTRY, 1964, 3 (05) :723-+

[2]

FLODIN P, 1963, DEXTRAN GELS THEIR A, P42

[3] STUDIES ON GEL FILTRATION - SORPTION PROPERTIES OF THE BED MATERIAL SEPHADEX [J].

GELOTTE, B .

JOURNAL OF CHROMATOGRAPHY, 1960, 3 (04) :330-342

[4] A SECOND VITAMIN B12-BINDING SUBSTANCE IN HUMAN PLASMA [J].

HALL, CA ;

FINKLER, AE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1963, 78 (01) :234-&

[5] MOLECULAR WEIGHTS OF VITAMIN B12 BINDING PROTEINS IN HUMAN SERUM DETERMINED BY SEPHADEX G-200 GEL FILTRATION [J].

HOM, B ;

OLESEN, H .

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1967, 19 (03) :269-&

[6]

JOUSTRA MARIUS K., 1967, PROTIDES BIOL FLUIDS, V14, P533

[7]

MARTIN RG, 1961, J BIOL CHEM, V236, P1372

[8] GEL FILTRATION OF ALBUMIN ON SEPHADEX G-200 IN UREA [J].

OLESEN, H ;

PEDERSEN, PO .

ACTA CHEMICA SCANDINAVICA, 1968, 22 (05) :1386-+

[9] GEL FILTRATION - METHOD FOR DESALTING AND GROUP SEPARATION [J].

PORATH, J ;

FLODIN, P .

NATURE, 1959, 183 (4676) :1657-1659

[10] DETERMINATION OF MOLECULAR WEIGHTS AND FRICTIONAL RATIOS OF PROTEINS IN IMPURE SYSTEMS BY USE OF GEL FILTRATION AND DENSITY GRADIENT CENTRIFUGATION . APPLICATION TO CRUDE PREPARATIONS OF SULFITE AND HYDROXYLAMINE REDUCTASES [J].

SIEGEL, LM ;

MONTY, KJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 112 (02) :346-+