NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF A GENE CONFERRING RESISTANCE TO ETHIONINE IN YEAST SACCHAROMYCES-CEREVISIAE

被引：31

作者：

SHIOMI, N

FUKUDA, H

FUKUDA, Y

MURATA, K

KIMURA, A

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,UJI,KYOTO 611,JAPAN

[2] KANEGAFUCHI CHEM IND CO LTD,ENGN RES LABS,TAKASAGO,HYOGO 676,JAPAN

[3] CHUKYO COMMUNITY COLL,MIZUNAMI,GIFU 50961,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 71卷 / 04期

关键词：

D O I：

10.1016/0922-338X(91)90269-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequence of a DNA fragment, when present on a multi-copy plasmid, conferring ethionine resistance to Saccharomyces cerevisiae cells was determined. The fragment contained one long open reading frame and the frame was confirmed to be an ethionine resistant gene caused by frame-shift mutation. Other than ethionine resistance, an increased dosage of the gene directed overaccumulation in cells of S-adenosyl-L-methionine. The protein deduced from the open reading frame consisted of 617 amino acid residues with a calculated molecular weight of 67, 977.

引用

页码：211 / 215

页数：5

共 20 条

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