N-LAURYLBIOTINAMIDE AS AFFINITY SURFACTANT

被引：34

作者：

COUGHLIN, RW

BACLASKI, JB

机构：

[1] Department of Chemical Engineering and Biotechnology Center, University of Connecticut, Storrs, Connecticut

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 04期

关键词：

D O I：

10.1021/bp00004a012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

N‐laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N‐laurylamine via the biotin ester of N‐hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse‐micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n‐octyl β‐D‐glucopyranoside. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：307 / 309

页数：3

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