FLUOROMETRIC-DETERMINATION OF OXIDIZED AND REDUCED GLUTATHIONE IN CELLS AND TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOLLOWING DERIVATIZATION WITH DANSYL CHLORIDE

被引：115

作者：

MARTIN, J

WHITE, INH

机构：

[1] MRC Toxicology Unit, Carshalton, Surrey SM5 4EF, Woodmansterne Road

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80356-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method utilising fluorimetric detection of oxidised and reduced glutathione, following derivatization with dansyl chloride is described. Dansyl derivatives are separated on an aminopropyl silica column with a methanol-sodium acetate gradient system giving detection limits (signal-to-noise ratio = 2) of 1 pmol. This is in the order of 100-fold more sensitive than established methods based on the ultraviolet detection of dinitrophenylglutathione derivatives. The present procedures have been used to determine oxidised and reduced glutathione in rat lung tissues and in alveolar macrophages.

引用

页码：219 / 225

页数：7

共 12 条

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