RAT-LIVER CANALICULAR MEMBRANE-VESICLES CONTAIN AN ATP-DEPENDENT BILE-ACID TRANSPORT-SYSTEM

被引:220
作者
NISHIDA, T [1 ]
GATMAITAN, Z [1 ]
CHE, MX [1 ]
ARIAS, IM [1 ]
机构
[1] TUFTS UNIV,SCH MED,DEPT PHYSIOL,BOSTON,MA 02111
关键词
D O I
10.1073/pnas.88.15.6590
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The secretion of bile by the liver is primarily determined by the ability of the hepatocyte to transport bile acids into the bile canaliculus. A carrier-mediated process for the transport of taurocholate, the major bile acid in humans and rats, was previously demonstrated in canalicular membrane vesicles from rat liver. This process is driven by an outside-positive membrane potential that is, however, insufficient to explain the large bile acid concentration gradient between the hepatocyte and bile. In this study, we describe an ATP-dependent transport system for taurocholate in inside-out canalicular membrane vesicles from rat liver. The transport system is saturable, temperature-dependent, osmotically sensitive, specifically requires ATP, and does not function in sinusoidal membrane vesicles and right side-out canalicular membrane vesicles. Transport was inhibited by other bile acids but not by substrates for the previously demonstrated ATP-dependent canalicular transport systems for organic cations or nonbile acid organic anions. Defects in ATP-dependent canalicular transport of bile acids may contribute to reduced bile secretion (cholestasis) in various developmental, inheritable, and acquired disorders.
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页码:6590 / 6594
页数:5
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