INCOMPLETE DEVELOPMENT OF HUMAN SPERMATOZOA IS ASSOCIATED WITH INCREASED CREATINE-PHOSPHOKINASE CONCENTRATION AND ABNORMAL HEAD MORPHOLOGY

被引:109
作者
HUSZAR, G
VIGUE, L
机构
[1] Sperm Physiology Laboratory, Department of Ob, Yale University School of Medicine, New Haven, Connecticut
关键词
CK ACTIVITY; CK CONCENTRATION; CK IMMUNOCYTOCHEMISTRY; SPERM MORPHOMETRY; FAILED SPERMATOGENESIS;
D O I
10.1002/mrd.1080340309
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our previous creatine phosphokinase (CK) activity studies in human sperm revealed differences among men and among sperm populations within the same specimen. Samples with low sperm concentrations, high incidence of abnormal sperm morphology, and diminished fertility had higher per sperm CK activity. In the present work, we demonstrated, with C-14-FDNB covalent CK active site modification and with direct CK immunocytochemistry, that the higher CK activity is related to an increased content of CK and of other proteins in sperm. Also, sperm heads with higher CK content were significantly larger and rounder and showed a higher incidence of amorph configuration. We suggest that these biochemical and morphological irregularities are related and are due to a failure of spermatogenesis, more specifically, to a higher retention of cytoplasm, which in normal sperm development is lost to the Sertoli cells as residual bodies. Thus higher CK activity and larger or irregular head size in human sperm signify cellular immaturity and a failure to complete spermatogenesis.
引用
收藏
页码:292 / 298
页数:7
相关论文
共 22 条