PDBLET, A STABLE AUTONOMOUSLY REPLICATING SHUTTLE VECTOR FOR SCHIZOSACCHAROMYCES-POMBE

被引：46

作者：

BRUN, C ^{[1
]}

DUBEY, DD ^{[1
]}

HUBERMAN, JA ^{[1
]}

机构：

[1] ROSWELL PK CANC INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263

来源：

GENE | 1995年 / 164卷 / 01期

关键词：

ARS; REPLICATION ORIGIN; PLASMID; CLONING; FISSION YEAST;

D O I：

10.1016/0378-1119(95)00497-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selectable marker and a new highly efficient ARS (autonomously replicating sequence) element, allowing the vector to remain stable as a monomer in Sp. In addition, pDblet transforms Sp with high efficiency and has high mitotic stability and low copy number.

引用

页码：173 / 177

页数：5

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