SIMULTANEOUS DNA TYPING OF HUMAN PLATELET ANTIGEN-2, ANTIGEN-3 AND ANTIGEN-4 BY AN ALLELE-SPECIFIC PCR METHOD

被引：44

作者：

TANAKA, S ^{[1
]}

TANIUE, A ^{[1
]}

NAGAO, N ^{[1
]}

OHNOKI, S ^{[1
]}

SHIBATA, H ^{[1
]}

OKUBO, Y ^{[1
]}

YAMAGUCHI, H ^{[1
]}

机构：

[1] OSAKA RED CROSS BLOOD CTR,OSAKA,JAPAN

来源：

VOX SANGUINIS | 1995年 / 68卷 / 04期

关键词：

D O I：

10.1111/j.1423-0410.1995.tb02577.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We developed an allele-specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The results were compared with those obtained by PCR restriction fragment length polymorphism and the mixed passive hemagglutination test. Seventy-three individuals were tested and the ASPCR results were in good agreement with those determined by the other two methods. This method enables the genotyping of HPA-2, -3 and -4 in parallel without the use of platelets, platelet-specific alloantibodies or restriction enzymes.

引用

页码：225 / 230

页数：6

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