STRUCTURE DETERMINATION OF THE DISIALYLATED POLY-(N-ACETYLLACTOSAMINE)-CONTAINING O-LINKED CARBOHYDRATE CHAINS OF EQUINE CHORIONIC-GONADOTROPIN

被引:24
作者
HOKKE, CH [1 ]
ROOSENBOOM, MJH [1 ]
THOMASOATES, JE [1 ]
KAMERLING, JP [1 ]
VLIEGENTHART, JFG [1 ]
机构
[1] UNIV UTRECHT,DEPT MASS SPECTROMETRY,3508 TB UTRECHT,NETHERLANDS
关键词
EQUINE CHORIONIC GONADOTROPIN; POLY-(N-ACETYLLACTOSAMINE); O-LINKED CARBOHYDRATE CHAINS;
D O I
10.1007/BF00732430
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The disialylated poly-(N-acetyllactosamine)-containing O-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymically N-deglycosylated beta-subunit of equine chorionic chonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and H-1-NMR spectroscopy. The identified oligosaccharide alditols have the following structure: [GRAPHICS] ]
引用
收藏
页码:35 / 41
页数:7
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