DIFFERENCES IN CA2+ PERMEABILITY OF AMPA-TYPE GLUTAMATE-RECEPTOR CHANNELS IN NEOCORTICAL NEURONS CAUSED BY DIFFERENTIAL GLUR-B SUBUNIT EXPRESSION

被引：512

作者：

JONAS, P ^{[1
]}

RACCA, C ^{[1
]}

SAKMANN, B ^{[1
]}

SEEBURG, PH ^{[1
]}

MONYER, H ^{[1
]}

机构：

[1] UNIV HEIDELBERG, ZENTRUM MOLEK BIOL, D-69120 HEIDELBERG, GERMANY

来源：

NEURON | 1994年 / 12卷 / 06期

关键词：

D O I：

10.1016/0896-6273(94)90444-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Fast excitatory synaptic transmission in the CNS is mediated by AMPA-type glutamate receptor (GluR) channels. Heterologous expression suggested that the Ca2+ permeability of these receptors critically depends on the subunit composition. Using patch-clamp techniques in brain slices, we found that the Ca2+ permeability of native AMPA-type GluRs was markedly higher in nonpyramidal (P-Ca/P-K approximate to 0.63) than in pyramidal (P-Ca/P-K approximate to 0.05) neurons of rat neocortex. Analysis of mRNA in single cells indicated that the relative abundance of GluR-B-specific mRNA was significantly lower in nonpyramidal (GluR-B/ GluR-non-B approximate to 0.3) than in pyramidal (GlUR-B/GluR-non-B approximate to 3) cells. This suggests that differences in relative abundance of GluR-B-specific mRNA generate functional diversity of AMPA-type GluRs in neurons with respect to Ca2+ permeability.

引用

页码：1281 / 1289

页数：9

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